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This book comprises a collection of chapters describing topics from traditional radiation cytogenetic analysis methods to the modern fluorescence based-analysis and high throughput automatic analysis methods. Cytogenetic analysis is important not only in order to understand radiation-induced DNA damage in cells, but it is also a standard biological method to estimate human radiation exposure in the radiation accidents. Therefore, we prepared a series of chapters not only covering biological aspects but also exploring radiation physics and radiation protection. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and equipment, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Radiation Cytogenetics: Methods and Protocols serves both novices working on their first experiments as well as experienced researchers searching for tried-and-true techniques to adapt for their own laboratory.


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An introductory discussion of basic chromosome structure and function preceeds the main text on the application of cytogenetic approaches to the analysis of the manipulation...


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The aim of this book is to provide a uniquely comprehensive source of information on the entire field of radiation therapy physics. The very significant advances in imaging,...


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